Combat acne throughout all 3 stages of acne pathogenesis

January 20, 2020

0.5% of ACZERO is the Minimum Inhibitory Concentration (MIC) that prevents visible growth of Propionibacterium acnes.

How does acne form? How to remove it?

Aczero

Acne is a common inflammatory skin disease that affects the pilosebaceous units of the skin. Without proper treatment, patients may suffer from severe skin scarring. Therefore, it has been researched extensively to develop potential treatment options.

Acne develops in 3 steps:

Step 1: High concentrations of sebum and keratinous material cause clogging of pores and in turn result in the formation of black and whitehead comedones.

Step 2: Whitehead and blackhead comedones are anaerobic, lipid-rich environments that are conducive for Propionibacterium acnes proliferation.

Step 3: Bacterial colonization stimulates the generation of chemotactic agents which leads to the formation of inflammatory lesions like papules, pustules and nodules or cysts.

Combination therapy that targets multiple acne development stages simultaneously has been receiving considerable attention due to its higher efficacy compared to monotherapy. MahaChem provides a natural anti-acne solution, ACZERO that targets all 3 stages of acne pathogenesis. Continue reading to find out more!

Our Solution: ACZERO

ACZERO is a natural solution for acne that comprises of 7 plant extracts:

No.	INCI Name	Function
1	Salix Alba (Willow) Bark Extract	Anti-inflammatory
2	Camellia Sinensis Leaf Extract	Anti-inflammatory, Anti-oxidative
3	Centella Asiatica Leaf Extract	Anti-oxidative, Anti-microbial
4	Cladosiphon Okamuranus Extract	Anti-microbial
5	Coffea Arabica (Coffee) Seed Extract	Anti-oxidative
6	Citrus Aurantium Bergamia (Bergamot) Leaf Extract	Anti-inflammatory
7	Pinus Densiflora Leaf Extract	Anti-microbial

What is so special about ACZERO?

Patented in Korea
Contains 7 plant extracts in optimal amounts to maximize the efficacy of acne treatment
Targets acne throughout its pathogenesis with a 3-step acne inhibition mechanism
Results in 4 weeks with low dosage used (2%)

ACZERO’s Anti-Acne Mechanism

Step 1: Inhibit proliferation of sebocytes, suppressing sebum production (Sebocytes are highly specialized, sebum-producing epithelial cells)

Step 2: Prevents bacterial colonization by inhibiting P.acne activity.

Step 3: Suppress inflammation by reducing inflammatory cytokine, IL-6 production.

Test and Studies on ACZERO

In-vitro tests proved that ACZERO is able to inhibit the proliferation of sebocytes and has higher efficacy compared to 13-Cis-retinoic acid which is an extremely effective drug for acne lesions! Furthermore, only 2% of ACZERO is required for significant results!

0.5% of ACZERO is the Minimum Inhibitory Concentration (MIC) that prevents visible growth of Propionibacterium acnes.

ACZERO is able to suppress inflammation through the reduction of IL-6. IL-6 is a pro-inflammatory cytokine that is strongly expressed in sebaceous glands of skin with acne and barely in healthy skin.

DPPH is used to measure the reducing ability of anti-oxidants towards the DPPH radical. ACZERO is shown to possess anti-oxidative properties.

According to the toxicity test, the addition of ACZERO has minimal effect on the percentage of cell viability. Thus, ACZERO is non-toxic to human skin.

In-Vivo Test Results

Further clinical testing on efficacy and safety was performed.

For efficacy testing, an in-vivo test was conducted on 11 participants at a dosage of 2%. As seen below, sebum secretion is reduced significantly.

Patch test was carried out on 31 participants and the results indicate that even with a dosage of 4%, ACZERO will not trigger skin irritation.

Formulation Guide

Application: Super mild facial wash with natural anti acne solution to prevent microorganism caused acne

Phase	Material	Function	INCI Name	Supplier	% w/w
A	Evermild GLDA-30	Chelating Agent	Tetrasodium Glutamate Diacetate	Sino Lion	0.1
	Deionized Water	Solvent	Deionized Water	-	Add to 100.0
	Refined Glycerine	Humectant	Glycerine	Vance	8.0
	Propylene Glycol	Humectant	Propylene Glycol	Dow Chemical	3.0
	Acecare 30 KC	Thickener	Acrylate Copolymer	KCI	8.0
B	NaOH (aq)	pH Adjuster	Pottasium Hydroxide, water	-	q.s.
C	Eversoft YCS 30 S	Surfactant	Sodium Cocoyl Glycinate	Sino Lion	5.0
D	Aczero	Antimicrobial,Anti oxidant, anti inflammation	Salix alba (willow) bark extract, Camellia Sinensis leaf extract, Centella asiatica leaf extract, Cladosiphon Okamuranus extract, Coffe arabica (coffe) seed extract, Citrus aurantium bergamia (Bergamot) leaf extract, Pinus Densiflora extract, Decyl glucoside, 1,2-Hexanediol	TEN	1.0
Troycare PE 91	Preservative	Phenoxyethanol, Ethylhexyl Glycerine	Troy	0.8

Procedures

Predisperse phase A in room temperature
Adjust pH to the range 6.5 – 7.5 with NaOH solution
Add phase C and continue mixing until homogeneous
Add phase D & E step by step while mixing

Interested to try ACZERO?

Email us for FREE sample now!

End Product Properties

Property	Results
Appearance	Gel, Clear-Slightly hazy
pH	6.98
Viscosity, cps	4706.6 mPa.s (25℃, Brookfield RVT, 18rpm, 3#spindle)